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Pamg 1 protein detection

The PartoSure® test, which detects the placental protein vaginal alphamicroglobulin-1 (PAMG-1), has satisfactory metrological qualities according to observational studies : NPV 98% and PPV 75% of delivery within 7 days. However this test has never been evaluated in real clinical condition The PartoSure test is used to detect the presence of placental alpha microglobulin-1 (PAMG-1), a protein present in the amniotic fluid throughout pregnancy. PAMG-1's presence in the vaginal. PAMG-1 was accurate (98%) for detection of PROM than Ferning (81.33%) or Nitrazine (84.0%) tests. Conclusion: Detection of the PAMG-1 in the vaginal fluid using AmniSure(®) test is an accurate method to diagnose rupture of the fetal membranes, with high sensitivity, specificity, negative and positive predictive values The novel kit for the detection of PAMG-1 is a diagnostic device employing monoclonal antibodies that detect Placental α1 Microglobulin present in cervico-vaginal secretions manufactured by AmniSure® International, LLC, Boston, MA. The in vivo sensitivity detection threshold is 4ng/ml Placental alpha microglobulin-1 (PAMG-1) is a human protein that was first isolated in 1975 from amniotic fluid.PAMG-1 is an important biomarker for the detection of premature rupture of fetal membrane (PROM) The high concentration of PAMG-1 in amniotic fluid means it can be used to detect if this fluid is present in the cervico-vaginal discharge of pregnant women; the presence of PAMG-1 in.

Evaluation of Vaginal PAMG-1 Detection by PartoSure Test

The positive predictive values of PAMG-1, fFN and CLM were 73.3%, 82.3%, and 64.2%, respectively, and the negative predictive values of PAMG-1, fFN and CLM were 92.9%, 90.9%, and 86.2%, respectively. CONCLUSION: The diagnostic accuracy of PAMG-1 is similar to that of fFN in terms of preterm labour detection within 7 days. PMCID: PMC5530467 PMID Placental alpha microglobulin-1 (PAMG-1) is being investigated as a marker for the detection of ROM. PAMG-1 may be found in high levels in amniotic fluid and low levels in cervicovaginal discharge when fetal membranes are intact. AmniSure ROM is an example of a PAMG-1 test. A sample of secretions are obtained via a vaginal swa The protein Placenta Alpha Microglobulin-1 (PAMG-1) is found in high concentrations in amniotic fluid. The PartoSure® test is an immunochromatographic test designed to detect PAMG-1 in vaginal secretions when preterm delivery is imminent . This test uses monoclonal antibodies with the sensitivity to detect 4 ng/µL of PAMG-1 in vaginal secretions

PartoSure Test - P160052 FD

  1. To investigate the utility of vaginal placental alpha microglobulin-1 (PAMG-1) protein as a predictor of preterm delivery within 7 days in pregnancies at risk of premature birth. This prospective study was performed in women at risk of premature birth. The levels of vaginal PAMG-1 and foetal fibronectin (fFN) and the transvaginal cervical length measurement (CLM) were investigated and compared
  2. AmniSure test kit is for the in vitro detection of human amniotic fluid PAMG-1 protein i vaginal secretion of pregnant women. The test should be used to clinical signs/symptoms suggestive of fetal membranes rupture. Results should always be used in conjunction with other clinical information
  3. AmniSure detects PAMG-1 (placental alpha-1 microglobulin) protein marker of the amniotic fluid in vaginal secretions and is intended to aid in the detection of ROM in pregnant women greater than 34 weeks gestation with signs, symptoms or complaints suggestive of ROM
  4. The AmniSure ROM (Rupture Of [fetal] Membranes) Test is a rapid, non-instrumented, qualitative immunochromatographic test for the in vitro detection of amniotic fluid in vaginal discharge of pregnant women. The AmniSure ROM Test uses amniotic fluid test strips to detect the PAMG-1 protein marker of the amniotic fluid in vaginal discharge
  5. Rapid, qualitative test for the in-vitro detection of amniotic fluid in vaginal discharge of pregnant women. Clinical Significance The Amnisure ROM test detects placental alpha-microglobulin-1 ( PAMG -1) protein marker of the amniotic fluid
  6. AmniSure detects PAMG-1 protein marker of the amniotic fluid in vaginal secretions. The test is for use by health care professionals to aid in the detection of rupture of membranes (ROM) in pregnant women at > 34 weeks gestation when patients report signs, symptoms or complaints suggestive of ROM (FDA, 2004)

AmniSure test kit is for the in vitro detection of human amniotic fluid PAMG-1 protein in vaginal secretion of pregnant women. The test should be used to evaluate patients with clinical signs/symptoms suggestive of fetal membranes rupture Detection limit and consistency were similar for both tests at all dilution. Actim PROM reaction time was shorter than AmniSure at all dilutions, except 1:320 (p<0.05). Conclusions: PAMG-1 and IGFBP-1 can be detected in amniotic fluid between 15 and 20 completed WG, using respectively AmniSure and actim PROM

AmniSure detects PAMG-1 protein marker of the amniotic fluid in vaginal secretions. The test is for use by health care professionals to aid in the detection of ROM in pregnant women at > 34 weeks gestation when patients report signs, symptoms or complaints suggestive of ROM. The Human freeze-dried PAMG-1 (amniotic fluid protein control) is a protein-1 (IGFBP-1, aka placental protein 12) and placental alpha microglobulin-1 (PAMG-1). 16-21. Enthusiasm about this point-of-care approach for the more accurate diagnosis of ROM has led to the recent development of a combined monoclonal/ polyclonal antibody immunoassay to detect two different pro In cervicovaginal secretion, PAMG-1 concentration at term is lower (0.05 to 0.22 ng/mL) than the AmniSure detection limit (5 ng/mL) whereas IGFBP-1 concentrations vary from 0.5 to 90 ng/mL and the detection limit of actim PROM test is 25 ng/mL. This could affect test result in clinical situation membranes. These proteins include placental alpha-1 microglobulin-1 (PAMG-1), placental protein 12 (PP12), alpha-fetoprotein (AFP), and insulin-like growth factor binding protein 1 (IGFBP-1). The tests are marketed for use by health care professionals to aid in the detection

1. J Obstet Gynaecol Res. 2017 Aug;43(8):1263-1269. doi: 10.1111/jog.13366. Epub 2017 Jun 14. Combined value of placental alpha microglobulin-1 detection and cervical length via transvaginal ultrasound in the diagnosis of preterm labor in symptomatic patients The AmniSure ROM Test detects PAMG-1 protein marker of the amniotic fluid in vaginal discharge. The test is for use by health care professionals (*Rx ONLY) to aid in the detection of ROM in pregnant women reporting signs, symptoms, or complaints suggestive of ROM. SUMMARY AND EXPLANATION OF THE TEST The timely and accurate diagnosis of rupture. PAMG-1 protein Reading/Result Visually-read qualitative test Same Detection limits Detects PP-12 at 5 ng/mL and AFP at 150 ng/mL Detects PAMG-1 at 5-7 ng/mL. Methodology Lateral flow immunochromatographic method same Timing mechanism Integrated timer External timer. Control. Detection of PAMG-1 Protein Marker in vaginal secretions. Performance. Testing is performed Monday through Sunday. Typically completed within 15 minutes after the specimen arrives at the testing site. CPT Code Information. 84112 Chemistry: Evaluation of cervicovaginal fluid for specfic amniotic fluid protein(s) (PAMG-1), each specimen

Placental alpha microglobulin-1 (AmniSure(®) test) for

  1. This study was designed to compare the accuracy of placental alpha microglobulin-1 (PAMG-1) versus insulin-like growth factor binding protein-1 (IGFBP-1) to diagnose premature rupture of fetal membranes (PROM). Material and Methods. This comparative prospective study was carried out over 3 years in Al-Rashid Maternity and Ahmadi Hospitals, Kuwait
  2. Recently, a bedside immunoassay (AmniSure rapid immunoassay) has been used to detect fetal glycoprotein, placental alpha microglobulin-1(PAMG-1), and in cervicovaginal secretions . Placental alpha microglobulin-1 is considered an ideal substance to be used for detection of ROM
  3. Previous studies suggested that PAMG-1 and IGFBP-1 detection in the vaginal fluid would be more accurate in the diagnosis of PROM than the current diagnostic methods. [7] , [12] , [14] , [15] Recently, a new, rapid immunoassay test (AmnioQuick ® Duo + ) test can detect two markers (IGFBP-1 and AFP) present in the amniotic fluid

Our objective was to compare two rapid strip tests for the detection of amniotic fluid, based on the detection of insulin-like growth factor-binding protein-1 (IGFBP-1) and of placental alpha-microglobulin-1 (PAMG-1). Samples of amniotic fluid were taken in 20 pregnant women between 31 3/7 and 41 2/7 gestational weeks at elective cesarean. PAMG-1 is a protein present in the amniotic cavity throughout pregnancy. Detection of PAMG-1 in the vagina in a patient with unruptured membranes is associated with an increased likelihood of. In this observational study on diagnostic test accuracy, we compared the performance of vaginal PAMG-1 protein detection with that of fFN and CLM in terms of delivery within 7 days. According to our results, the diagnostic accuracy of PAMG-1 regarding delivery within 7 days is similar to that of fFN Negative AmniSure is an immunochromatographic assay for PAMG-1, an amniotic fluid protein. Detection of PAMG-1 in vaginal fluid is evidence of ruptured fetal membranes. Results should be interpreted in conjunction with clinical and other laboratory findings The detection of rupture of fetal membranes with the nitrazine indicator. Am J Obstet Gynecol 1940; 39:400 and Davidson, KM. Detection of premature rupture of the membranes. which is a point of care immunochromatographic assay that detects trace amounts of placental alpha microglobulin-1 protein (PAMG-1) in vaginal fluid after rupture of.

Evaluation of a Novel Diagnostic Kit for the Detection of

AmniSure ROM Test (Rupture of [fetal] Membranes testStat-X Fire Suppression System - Animation Video - YouTubeChapter VBI-12

Objective: To compare PAMG-1 and phIGFBP-1 tests in predicting impending spontaneous preterm delivery within 7 days upon presentation in pregnant women with symptoms of preterm labor. Study Design: From September 2014 to April 2015 women with singleton gestation, symptoms of preterm labor, GA 22 - 35, participated in this prospective cohort study upon admission AmniSure test kit is for the in vitro detection of human amniotic fluid PAMG-1 protein in vaginal secretion of pregnant women. The test should be used to evaluate patients with clinical signs/symptoms suggestive of fetal membranes rupture. Hazards: Kit contents are for in vitro diagnostic use detection of PAMG-1 protein. Compatible with AmniSure ROM Test - 1 sample 1 mL serum - 2 samples 0.7 mL hemolysate - 2 samples (for Axis-Shield Afinion, see Cat. #134) 0.7 mL hemolysate - for laboratories that need more sample volume Designed for the Axis-Shield Afinion - 2 samples 1 mL whole blood 1 mL whole blood - 2 sample IGFBP-1 (AMNIOQuick ® DUO+) and PAMG-1 detection in the vaginal fluid showed to be more accurate in the diagnosis of PROM than the current diagnostic methods. Storage and stability of AMNIOQuick ® DUO+ AMNIOQuick ® DUO+ test is packed in aluminium pouch. The kit should be stored in a dry area at 2-30°C

CPT 84112 Evaluation of cervicovaginal fluid for specific amniotic fluid protein(s) (eg, placental alpha microglobulin-1 [PAMG-1], placental protein 12 [PP12], alpha-fetoprotein), qualitative, each specimen. *The national limit is $98.11; however, reimbursement amount may vary by state To improve identification among symptomatic women, methods such as cervical-length (CL) measurement and biochemical testing, including fetal fibronectin (fFN), placental alpha microglobulin-1 (PAMG-1) and cervical phosphorylated insulin-like growth factor binding protein-1 (phIGFBP-1) have been proposed 5 on IGFBP-1 and PAMG-1 for the Detection of Amniotic Fluid. Am J Perinatol 2008;(25):243-246. 4. Gaucherand P., Doret M., et al. Detection of Placental Alpha Microglobulin-1 versus Insulin-Like Growth Factor-Binding Protein-1 in Amniotic Fluid at Term: A Comparative Study. Am J Perinatol 2011;(6):489-94. 5 AmniSure detects PAMG-1 (placental alpha-1 microglobulin) protein marker of the amniotic fluid in vaginal secretions and is intended to aid in the detection of ROM in pregnant women greater than 34 weeks gestation with signs, symptoms or complaints suggestive o The AmniSure ROM Test detects PAMG-1 protein marker of the amniotic fluid in vaginal discharge. The test is for use by health care professionals (*Rx ONLY) to aid in the detection of ROM in pregnant women reporting signs, symptoms, or complaints suggestive of ROM. The timely and accurate diagnosis of rupture of [fetal] membranes (ROM) is.

Video: Placental alpha microglobulin-1 (PAMG-1) - Wikipedi

Use of cervicovaginal PAMG-1 protein as a predictor of

tography to detect human PAMG-1 protein present in amniotic fl uid. The test employs highly sensitive monoclonal antibodies that detect even a minimal amount of PAMG-1, which is present in cervicovaginal discharge after rupture of fetal membranes. PAMG-1 was selected as a marker of fetal membranes rupture du BMC Pregnancy and Childbirth (2017-07-01) . Use of cervicovaginal PAMG-1 protein as a predictor of delivery within seven days in pregnancies at risk of premature birt

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PAMG-1 is a protein released from the lining of the uterus into the amniotic cavity throughout pregnancy. It is found in very high concentrations in amniotic fluid and in very low concentrations in normal vaginal discharge. Studies have PAMG-1 in the sample exceeds the detection limit of the test (1 ng/ml) Doret, M. , Cartier, R. , Miribel, J. Premature preterm rupture of the membrane diagnosis in early pregnancy: PAMG-1 and IGFBP-1 detection in amniotic fluid with biochemical tests. Clin Biochem. 2013; 46(18): 1816 - 1819. Google Scholar | IS PAMG-1 (AmniSure®): placental alpha microglobulin-1 protein Rapid, noninstrumental, point of care immunochromatographic assay PAMG-1 is present in the blood, amniotic fluid and cervico-vaginal discharge of pregnant wome Our objective was to compare two rapid strip tests for the detection of amniotic fluid, based on the detection of insulin-like growth factor-binding protein-1 and of placental alpha-microglobulin-1 (PAMG-1).Samples of amniotic fluid were taken in 20 pregnant women between 31 3/7 and 41 2/7 gestational weeks at elective cesarean section before delivery of the newborn

Placental alpha microglobulin-1 (AmniSure test) versus insulin-like growth factor binding protein-1 (Actim PROM test) for detection of premature rupture of fetal membranes. - Ibrahim A Abdelazim, Hanan H Makhlou

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The AmniSure test measures the presence of placental alpha macroglobulin-1 (PAMG-1) protein in the amniotic fluid using an immunochromotographic assay from a vaginal swab. This test has been reported to have a high sensitivity for detecting the PAMG-1 protein. 2 However, th Abstract Objective: The PartoSure™ time-to-delivery (TTD) test (AmniSure International, Boston, MA, USA) is a newly available bedside test for the prediction of time to spontaneous preterm delivery via the detection of placental alpha microglobulin-1. The objective of this study was to determine the efficacy of the test in predicting imminent delivery in 7 or 14 days from the time of testing The positive predictive values of PAMG-1, fFN and CLM were 73.3%, 82.3%, and 64.2%, respectively, and the negative predictive values of PAMG-1, fFN and CLM were 92.9%, 90.9%, and 86.2%, respectively. Conclusion The diagnostic accuracy of PAMG-1 is similar to that of fFN in terms of preterm labour detection within 7 day AmniSure test kit is for the in vitro detection of human amniotic fluid PAMG-1 protein in vaginal secretion of pregnant women. The test should be used to evaluate patients with clinical signs/symptoms suggestive of fetal membranes rupture. Until the diagnosis of membrane rupture is excluded, avoid digital cervical examination to preven Technology Description: The AmniSure ROM Test (Rupture of [fetal] Membranes test) (Qiagen N.V., the Netherlands) has been developed to detect fetal membrane rupture based on the presence of placental alpha-microglobulin-1 protein (PAMG-1) in vaginal fluid. Controversy: Although the AmniSure test is relatively easy to perform and could enable.

The AmniSure test measures the presence of placental alpha macroglobulin-1 (PAMG-1) protein in the amniotic fluid using an immunochromotographic assay from a vaginal swab. This test has been reported to have a high sensitivity for detecting the PAMG-1 protein. 2. However, th The concentration of this protein in amniotic fluid is 1,000-10,000 times higher than in cervicovaginal fluid. 19 Consequently, the presence of high concentrations of PAMG-1 in cervicovaginal fluid is considered evidence of rupture of membranes, and the threshold of the test for the diagnosis has been set at 5.0 ng/mL. AmniSure test is not. Amnisure (PAMG-1 assay) is a bedside strip test with a sensitivity of 98.9%, specificity of 100%, a PPV of 100%, and an NPV of 99.1% , but its positive results should be interpreted cautiously, because the clinical significance of a positive test due to micro-leakage of amniotic fluid is not yet clear (15, 16)

The value of a vaginal sample for detecting PAMG-1

본 발명은 pamg-1 항체들을 탐지함으로써 질 내의 소량의 양수를 검출하는 진단 방법 및 장치에 관한 것이다. 본 발명 장치는, 도 1에 도시한 바와 같이, m271 이라는 칭하는 항체 영역(10), 테스트 영역(14), 및 제어 영역(16)을 구비하는 패드(12)를 포함한다 alpha macroglobulin-1 (PAMG-1). PAMG-1 is a protein present in the Detection of PAMG-1 in the vagina in a patient with unruptured membranes is associated with an increased like The evaluation of amnisure for the detection of premature rupture of membranes. MOJ Womens Health. 2015;1(1):7‒10. DOI: 10.15406/mojwh.2015.01.00003 amount of Placental Micro globulin (PAMG -1), which is an amniotic fluid protein that has been found to be present in cervico-vaginal secretions after the breakage of fetal membranes. AmniSure is. The PartoSure™ TTD Test detects placental alpha- microglobulin-1 (PAMG-1), a protein found in very high concentrations in amniotic fluid and in very low concentrations in normal vaginal discharge. Recent clinical studies have demonstrated a strong correlation between a positive PAMG-1 test and imminent delivery in those patients presenting.

Non-Invasive Fetal Membranes Rupture Tests - Medical

AmniSure ROM Test (Rupture of [fetal] Membranes test

PartoSure is a rapid, qualitative test for detecting the presence of placental alpha microglobulin-1 (PAMG-1) in cervicovaginal secretions in pregnant women with signs and symptoms of early preterm labor (7). PAMG-1 is a placental protein found in high concentrations in the amniotic cavity. Due to the low concentratio Pollet-Villard M, Cartier R, Gaucherand P, Doret M. Detection of placental alpha microglobulin-1 versus insulin-like growth factor-binding protein-1 in amniotic fluid at term: a comparative study. Am J Perinatol 2011; 28: 489-494

Results: PAMG-1 detection in cervico vaginal discharge was a very good test for diagnosis of PROM with high sensitivity, specificity, positive predictive value, negative predictive value, accuracy and P-value (96%, 100%, 100%, 95.84%, 97.78% and diagnosis of PROM with high sensitivity, and specificity after PAMG-1 with a privilege of low cost. The AmniSure test is for the in vitro detection of human amniotic fluid PAMG-1 protein in vaginal secretion of pregnant women. The test should be used to evaluate patients with clinical signs/symptoms suggestive of fetal membranes rupture. 2. No quantitative interpretation should be made based on the test results. 3 The pooled DOR of the PAMG-1 for prediction delivery within 14 days of testing was equal to 44.65 (95 % CI: 26.30 to 75.78). The authors concluded that cervical PAMG-1 had a high accuracy to predict PB within 7 and 14 days of testing in symptomatic pregnant women

ALPHA 1-MICROGLOBULIN (AMNISURE ROM) - NorDx Test Catalo

OBJECTIVE To evaluate the presence of placental α-microglobulin-1 (PAMG-1) in vaginal secretions in women with symptoms of preterm labor and assess its use as a predictor of preterm birth. STUDY DESIGN A prospective cohort study of women between 16 and 34 weeks of gestation with symptoms of preterm labor and intact membranes was conducted. The presence of PAMG-1 was determined using a. PAMG-1 detection was more reliable and non invasive than other methodologies and that (ii) the rapid strip test based on PAMG-1 no evidence to promote the use of PAMG-1. Moreover, IGFBP-1 is a protein that has been fully characterised and that can be quanti-fied using different immunoassay. This is not the case for PAMG-1 that the protein concentration levels in the fluid samples were all below the upper limits given for the three test kits. The threshold for lower limit of detection for the kits are as follows: Actim™PROM =25ng/ml IGFBP-1, AmniSure® =5 ng/ml PAMG-1, ROM +plus® = 5ng/ml IGFBP-1,and 150 ng/ml AFP. As shown in Table 1 and 2, all amniotic fluid. The Amnisure ROM immunoassay for PAMG-1 has a lower limit of detection of 5 ng/ml in cervicovaginal secretions, and may therefore, be more sensitive to detect subclinical PROM from small perforations (which may account for equivocal cases), which subsequently reduces the chances of obtaining false positive or negative results Bolotskikh Viacheslav (RU) The role of PAMG-1 protein and ultrasound cervicometry in detection of spontaneous preterm labour; bedoui olfa Unconsummated Marriage in the Arab Islamic World: Tunisian Experience; bedoui olfa Comparative study of disorders of sexual function post partum in primiparous women

LAB.00029 Rupture of Membranes Testing in Pregnanc

and PartoSure™ share the same characteristics and method for detecting the PAMG-1 protein marker of amniotic fluid in vaginal discharge. AmniSure ® ROM was However, developed for a different patient group in the pathway. ® AmniSure. is for the detection of ROM in pregnant women reporting signs and symptoms suggestive of ROM. Technology . ALER Evaluation of cervicovaginal fluid for specific amniotic fluid protein(s) (eg, placental alpha microglobulin-1 [PAMG-1], placental protein 12 [PP12], alpha-fetoprotein), qualitative, each specimen: Clinical notes: 84181: Protein; Western Blot, with interpretation and report, blood or other body fluid: Clinical notes and invoice: 8748 The present invention relates to a diagnostic method and device for the detection of small quantities of amniotic fluid in the vagina by detecting PAMG-1 antibodies. The device, as shown in Figure 1, comprises a pad (12) which contains labeled M271 antibody region (10), a test region (14), and a control region (16) Diagnosis of ruptured fetal membranes is of crucial importance at any period in a pregnancy for prompt hospitalization and for timely and proper treatment. The AmniSure ROM Test: Is ~99% accurate*. Detects the PAMG-1 protein marker. Provides results within minutes. Does not require a speculum examination The 4-step test procedure detects placental alpha microglobulin-1 (PAMG-1) protein that is found in high concentrations in amniotic fluid and low concentrations in cervicovaginal fluid (5). • Saves time and costs of additional PROM diagnostic method

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Premature preterm rupture of the membrane diagnosis in

Doret M, Cartier R, Miribel J, et al. Premature preterm rupture of the membrane diagnosis in early pregnancy: PAMG-1 and IGFBP-1 detection in amniotic fluid with biochemical tests. Clin Biochem. 2013;46(18):1816-1819. 6 Clinical studies suggest that PAMG-1 based tests provide improved diagnostic accuracy and sensitivity compared to traditional tests for the detection of premature rupture of membranes. This key factor is poised to contribute to growth in the segment's sales in 2025

Combined value of placental alpha microglobulin-1

EP2577317B1 EP20110728343 EP11728343A EP2577317B1 EP 2577317 B1 EP2577317 B1 EP 2577317B1 EP 20110728343 EP20110728343 EP 20110728343 EP 11728343 A EP11728343 A EP 11728343A EP 2577317 B1 EP2577317 B1 EP 2577317B1 Authority EP European Patent Office Prior art keywords afp igfbp negative negative antibodies positive Prior art date 2010-06-03 Legal status (The legal status is an assumption and. Aim: The aim of this study was to compare the performance of tests based on the detection of insulin-like growth factor binding protein 1 (IGFBP-1) and placental α-microglobulin-1 (PAMG-1) in diagnosing rupture of fetal membranes (ROM) across different patient populations The mechanism of preterm labor is similar to term labor including uterine contractility, cervical ripening, and membrane rupture. Preterm labor can be predicted through cervical length measurement by transvaginal ultrasound, fetal fibronectin, and IGF binding protein-1 or placental alpha-microglobulin-1 (PAMG-1)

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AIM: The aim of this study was to compare the accuracy of placental α-microglobulin-1 (PAMG-1), insulin-like growth factor binding protein-1 (IGFBP-1) and nitrazine test to diagnose premature rupture of membranes. MATERIAL AND METHODS: A total of 120 pregnant women between 11 and 42 weeks with signs/symptoms of membrane rupture were eligible for our study A cotton swab used to collect vaginal secretion specimens may affect the detection of (review table in powerpoint for other swabs and organisms affected) the test is negative for ROM An immunochromatographic assay using monoclonal antibodies to detect PAMG-1 observed to have a line at the control zone but not at the test zone on the test strip [lung], mass spec 8 -protein signature Gapfill 14 100%. 24. 0092U: Oncology (lung), three protein biomarkers, immunoassay using magnetic nanosensortechnology, plasma, algorithm reported as risk score for likelihood of malignancy Medicare Advisory Panel on Clinical Diagnostic Laboratory Tests Meeting (July 22 -23, 2019 Placental alpha macroglobulin-1 (PAMG-1) levels, fetal fibronectin levels, and ultrasound have been generally used to diagnose preterm birth [4,5].PAMG-1 is found in amniotic fluid during pregnancy, and its concentration is 1000 times higher than that in normal vaginal discharge or maternal blood [6,7].PAMG-1 can be detected using a lateral flow immunoassay, which are typically tests strips. Chen and Dudenhausen (2008) compared 2 rapid strip tests for the detection of amniotic fluid, based on the detection of insulin-like growth factor-binding protein-1 (IGFBP-1) and of PAMG-1. Samples of amniotic fluid were taken in 20 pregnant women between 31 3/7 and 41 2/7 gestational weeks at elective cesarean sectio